If you're treating the water before you add it to the tank, you treat for just that amount. If you are treating the tank and then adding water, you treat for the volume of the entire tank. Which is one of the reasons why I love Prime.
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Puffers:
Auriglobus silus x2, Tetraodon travancoricus x1, Tetraodon miurus x1, Tetraodon turgidus x1, Tetraodon nigroviridis x2, Tetraodon baileyi x2, Tetraodon lineatus x1, Tetraodon palembangensis x1
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