Water conditioners work instantly. Many of us use a product called a Python to do waterchanges by directly putting the water in the tank from the tap... I add conditioner when I start the water into the tank, while the fish are still in it...and have never had a problem. There does need to be some kind of filter media in the cannister, but I really don't know much about cannisters so I will allow someone else to answer that question.
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